CD47 Antibody (BRIC126) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42488

Clone BRIC126 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-42488
NBP1-42488-0.05mg
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Key Product Details

Species Reactivity

Human, Porcine, Bovine, Canine, Sheep

Applications

Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # BRIC126

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD47 Antibodies »

Product Specifications

Immunogen

Human erythrocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for CD47 Antibody (BRIC126) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
The antibody is very useful for flow cytometry (dilution 1/10), immunohistochemistry on frozen sections. With this antibody immunoprecipitations can be pre-formed.For flow cytometry use 10 ul of the suggested working dilution to label 106 cells in 100 ul. Concentration of the antibody is approximately 1.0 mg/ml.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-buffered glycine

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells. OX-101 (this clone) recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

Additional CD47 Products

Product Documents for CD47 Antibody (BRIC126) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD47 Antibody (BRIC126) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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