CD47 Antibody (CD47/3019)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08975

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CD47/3019

Concentration

0.2 mg/ml

View all CD47 Antibodies »

Product Summary for CD47 Antibody (CD47/3019)

Immunogen

A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary) (Uniprot: Q08722)

Localization

Cell Surface

Specificity

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08976)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for CD47 Antibody (CD47/3019)

Immunocytochemistry/ Immunofluorescence: CD47 Antibody (CD47/3019) [NBP3-08975]

Immunocytochemistry/ Immunofluorescence: CD47 Antibody (CD47/3019) [NBP3-08975]

Immunocytochemistry/Immunofluorescence: CD47 Antibody (CD47/3019) [NBP3-08975] - Immunofluorescence staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot.
Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975]

Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975]

Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Biotin-conjugated CD47 Mouse Monoclonal Antibody (IAP/3019).
Flow Cytometry: CD47 Antibody (CD47/3019) [NBP3-08975]

Flow Cytometry: CD47 Antibody (CD47/3019) [NBP3-08975]

Flow Cytometry: CD47 Antibody (CD47/3019) [NBP3-08975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
View 3 more images

Applications for CD47 Antibody (CD47/3019)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
ELISA: For coating, order without BSA
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

UniProt

Q08722

Additional CD47 Products

Product Documents for CD47 Antibody (CD47/3019)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD47 Antibody (CD47/3019)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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