CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47812

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin (Negative), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # IAP/964 + B6H12.2

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD47 Antibodies »

Product Summary for CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free

Immunogen

Recombinant human CD47 protein (CD47/964); Intact CD47 Purified from placenta (B6H12.2) (Uniprot: Q08722)

Localization

Cell surface, cytoplasmic

Specificity

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG1 Kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44409).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free [NBP2-47812]

Immunocytochemistry/ Immunofluorescence: CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free [NBP2-47812]

Immunocytochemistry/Immunofluorescence: CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free [NBP2-47812] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CD47 Antibody (IAP/964 + B6H12.2) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot.
Immunohistochemistry-Frozen: CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free [NBP2-47812]

Immunohistochemistry-Frozen: CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free [NBP2-47812]

Immunohistochemistry-Frozen: CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free [NBP2-47812] - Human frozen tonsil tissue. Antibody at 1:100. IHC-Fr image submitted from a verified customer review

Applications for CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free

Application
Recommended Usage

Flow (Cell Surface)

1-2ug/million cells in 0.1 ml

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunofluorescence

1 - 2 ug/ml

Western Blot

1-2ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
validated for IHC-Fr from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-47812 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

Additional CD47 Products

Product Documents for CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD47 Antibody (IAP/964 + B6H12.2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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