CD47 Antibody (miap301) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43407

Novus Biologicals, part of Bio-Techne
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NBP1-43407-0.5mg
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # miap301

Format

BSA Free

Concentration

0.5 mg/ml

View all CD47 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD47.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for CD47 Antibody (miap301) - BSA Free

Flow Cytometry: CD47 Antibody (miap301) [NBP1-43407]

Flow Cytometry: CD47 Antibody (miap301) [NBP1-43407]

Flow Cytometry: CD47 Antibody (miap301) [NBP1-43407] - Staining of BALB/c splenocytes.

Applications for CD47 Antibody (miap301) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 ug/10^6 cells
Application Notes
This miap30 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Aqueous , may contain carrier protein/stabilizer.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD47

The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with 3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells. No crossreactivity is seen against human CD47.

Alternate Names

CD47, IAP, MER6, OA3

Entrez Gene IDs

961 (Human)

Gene Symbol

CD47

UniProt

Q08722

Additional CD47 Products

Product Documents for CD47 Antibody (miap301) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD47 Antibody (miap301) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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