CD47 Antibody (SR1983)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21647

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21647-100ul
NBP3-21647-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1983

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD47 Antibodies »

Product Summary for CD47 Antibody (SR1983)

Immunogen

A synthesized peptide derived from human CD47 (Uniprot #: Q08722)

Specificity

Detects endogenous levels of total CD47

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD47 Antibody (SR1983)

Western Blot: CD47 Antibody (SR1983) [NBP3-21647] -

Western Blot: CD47 Antibody (SR1983) [NBP3-21647] - Western blot analysis of extracts of NIH/3T3 cell lysate, using NBP3-21647.

Applications for CD47 Antibody (SR1983)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

Additional CD47 Products

Product Documents for CD47 Antibody (SR1983)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD47 Antibody (SR1983)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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