CD5 Antibody (CD5/54/F6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34593
![Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]](https://resources.bio-techne.com/images/products/CD5-Antibody-CD5-54-F6-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34593-img0002.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Porcine, Bovine, Chicken, Equine, Guinea Pig, Monkey, Rabbit
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CD5/54/F6
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region). (Uniprot: P06127 )
Localization
Cell surface
Specificity
Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
Marker
Mantle Cell Lymphoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32824).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD5 Antibody (CD5/54/F6) - Azide and BSA Free
Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]
Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593] - Formalin-fixed, paraffin-embedded human tonsil stained with CD5 MAb (CD5/54/F6)![Flow Cytometry: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]](https://resources.bio-techne.com/images/products/CD5-Antibody-CD5-54-F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34593-img0001.jpg "Flow Cytometry: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]")
Flow Cytometry: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593]
Flow Cytometry: CD5 Antibody (CD5/54/F6) - Azide and BSA Free [NBP2-34593] - T cell characterization in canine whole blood cells. From a verified customer review. Image using the Alexa Fluor 700 format of this antibody.Applications for CD5 Antibody (CD5/54/F6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD5
Alternate Names
CD5
Gene Symbol
CD5
Additional CD5 Products
Product Documents for CD5 Antibody (CD5/54/F6) - Azide and BSA Free
Product Specific Notices for CD5 Antibody (CD5/54/F6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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