CD5 Antibody (CD5/54/F6) [PE/Atto594]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34593PEATT594
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Porcine, Bovine, Chicken, Equine, Guinea Pig, Monkey, Rabbit
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CD5/54/F6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region). (Uniprot: P06127 )
Localization
Cell surface
Specificity
Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
Marker
Mantle Cell Lymphoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for CD5 Antibody (CD5/54/F6) [PE/Atto594]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD5
Alternate Names
CD5
Gene Symbol
CD5
Additional CD5 Products
Product Documents for CD5 Antibody (CD5/54/F6) [PE/Atto594]
Product Specific Notices for CD5 Antibody (CD5/54/F6) [PE/Atto594]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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