CD5 Antibody (CL12334)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15133

Novus Biologicals, part of Bio-Techne
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NBP3-15133-100ul
NBP3-15133-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL12334

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD5 Antibodies »

Product Summary for CD5 Antibody (CL12334)

Immunogen

This antibody was developed against a synthetic peptide. Actual immunogen sequence is proprietary information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CD5 Antibody (CL12334)

Western Blot: CD5 Antibody (CL12334) [NBP3-15133]

Western Blot: CD5 Antibody (CL12334) [NBP3-15133]

Western Blot: CD5 Antibody (CL12334) [NBP3-15133] - Analysis in human cell line MOLT-4.
Immunocytochemistry/ Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133]

Immunocytochemistry/ Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133]

Immunocytochemistry/Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133] - Staining of A-431 cells using the Anti-CD5 monoclonal antibody, showing localization in plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively.
Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]

Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]

Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Analysis in human lymph node and skeletal muscle tissues using NBP3-15133 antibody. Corresponding CD5 RNA-seq data are presented for the same tissues.
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Applications for CD5 Antibody (CL12334)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000- 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2), 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD5

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Alternate Names

CD5

Gene Symbol

CD5

Additional CD5 Products

Product Documents for CD5 Antibody (CL12334)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD5 Antibody (CL12334)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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