CD5 Antibody (CL12420)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21215
![Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -](https://resources.bio-techne.com/images/products/nbp3-21215_mouse-cd5-mab-cl12420-4520231550323.jpg "Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL12420
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for CD5 Antibody (CL12420)
Immunogen
This antibody was developed against a Synthetic Peptide of P06127. Actual immunogen sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for CD5 Antibody (CL12420)
![Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -](https://resources.bio-techne.com/images/products/nbp3-21215_mouse-cd5-mab-cl12420-45202315524515.jpg "Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -")
Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -
Staining of human chronic lymphocytic leukemia shows strong membranous positivity in tumor cells.![Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -](https://resources.bio-techne.com/images/products/nbp3-21215_mouse-cd5-mab-cl12420-4520231548105.jpg "Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -")
Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -
Staining of human small intestine shows strong membranous positivity in lymphoid cells.![Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -](https://resources.bio-techne.com/images/products/nbp3-21215_mouse-cd5-mab-cl12420-45202315531370.jpg "Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -")
Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -
Staining of human skeletal muscle shows no positivity in myocytes as expected.![Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -](https://resources.bio-techne.com/images/products/nbp3-21215_mouse-cd5-mab-cl12420-45202315491421.jpg "Immunohistochemistry-Paraffin: CD5 Antibody [NBP3-21215] -")
![Western Blot: CD5 Antibody [NBP3-21215] -](https://resources.bio-techne.com/images/products/nbp3-21215_mouse-cd5-mab-cl12420-4520231552922.jpg "Western Blot: CD5 Antibody [NBP3-21215] -")
![Immunocytochemistry/Immunofluorescence: CD5 Antibody [NBP3-21215] -](https://resources.bio-techne.com/images/products/nbp3-21215_mouse-cd5-mab-cl12420-45202315493223.jpg "Immunocytochemistry/Immunofluorescence: CD5 Antibody [NBP3-21215] -")
Applications for CD5 Antibody (CL12420)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000- 1:2500
Immunohistochemistry-Paraffin
1:1000- 1:2500
Western Blot
1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD5
Alternate Names
CD5
Gene Symbol
CD5
Additional CD5 Products
Product Documents for CD5 Antibody (CL12420)
Product Specific Notices for CD5 Antibody (CL12420)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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