CD5 Antibody (PD00-01)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32158

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32158
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD00-01

Concentration

1 mg/ml

View all CD5 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CD5 aa 403 - 495 (intracellular). (Uniprot: P06127)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD5 Antibody (PD00-01)

CD5 Antibody (PD00-01)

Immunohistochemistry: CD5 Antibody (PD00-01) [NBP3-32158]

Immunohistochemistry: CD5 Antibody (PD00-01) [NBP3-32158] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD5 antibody (NBP3-32158) at 1/1,500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32158) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for CD5 Antibody (PD00-01)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD5

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Alternate Names

CD5

Gene Symbol

CD5

Additional CD5 Products

Product Documents for CD5 Antibody (PD00-01)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD5 Antibody (PD00-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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