Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all CD55/DAF Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF. The immunogen is located within amino acids 360 - 410 of DAF. Amino Acid Squence: DRSSLQSRTPGFKRSFH

Specificity

DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD55/DAF Antibody - BSA Free

Western Blot: CD55/DAF AntibodyBSA Free [NBP2-41295]

Western Blot: CD55/DAF AntibodyBSA Free [NBP2-41295]

Western Blot: CD55/DAF Antibody [NBP2-41295] - Western blot analysis of CD55/DAF expression in HeLa cell lysate with CD55/DAF antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry: CD55/DAF Antibody - BSA Free [NBP2-41295]

Immunohistochemistry: CD55/DAF Antibody - BSA Free [NBP2-41295]

Immunohistochemistry: CD55/DAF Antibody [NBP2-41295] - Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 ug/ml.
Western Blot: CD55/DAF AntibodyBSA Free [NBP2-41295]

Western Blot: CD55/DAF AntibodyBSA Free [NBP2-41295]

Western Blot: CD55/DAF Antibody [NBP2-41295] - Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 ug/ml.
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Applications for CD55/DAF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD55/DAF

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

CD55, CR, DAF, TC

Gene Symbol

CD55

Additional CD55/DAF Products

Product Documents for CD55/DAF Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD55/DAF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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