CD58/LFA-3 Antibody (085)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90096

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-90096-100ul
NBP2-90096-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 085

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD58/LFA-3 Antibodies »

Product Summary for CD58/LFA-3 Antibody (085)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD58/LFA-3 (Accession#: AAH05930.1; Met1-Arg215).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD58/LFA-3 Antibody (085)

Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096]

Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096]

Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: CD58/LFA-3 Antibody (085) [NBP2-90096]

Immunoprecipitation: CD58/LFA-3 Antibody (085) [NBP2-90096]

Immunoprecipitation: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CD58 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD58 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 61 kDa

Applications for CD58/LFA-3 Antibody (085)

Application
Recommended Usage

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD58/LFA-3

LFA3 is a type 1 transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed on most hematopoietic cells and on nonhematopoietic cells such as fibroblasts, endothelial and epithelial cells. Its expression is particularly high on memory T-cells and dendritic cells. LFA3 expression on antigen-presenting cells and target cells enhances T-cells antigen recognition through binding to its ligand, LFA2/CD2, on T-cells.

Alternate Names

CD58, LFA-3, LFA3

Gene Symbol

CD58

Additional CD58/LFA-3 Products

Product Documents for CD58/LFA-3 Antibody (085)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD58/LFA-3 Antibody (085)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.