CD59 Antibody (MEM-43/5) [Allophycocyanin/Cy7]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-400APCCY7
Clone MEM-43/5 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Flow Cytometry
Label
Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # MEM-43/5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD59 Antibody (MEM-43/5) [Allophycocyanin/Cy7]
Immunogen
Thymocytes and T lymphocytes.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies. HLDA V; WS Code AS S012
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for CD59 Antibody (MEM-43/5) [Allophycocyanin/Cy7]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD59
Alternate Names
CD59, HRF20, MACIF, MIC11, Protectin
Gene Symbol
CD59
Additional CD59 Products
Product Documents for CD59 Antibody (MEM-43/5) [Allophycocyanin/Cy7]
Product Specific Notices for CD59 Antibody (MEM-43/5) [Allophycocyanin/Cy7]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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