CD59 Antibody (MEM-43) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-330

Clone MEM-43 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB500-330
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-43

Format

BSA Free

Concentration

1 mg/ml

View all CD59 Antibodies »

Product Specifications

Immunogen

Thymocytes and T lymphocytes (NM_000611.5).

Specificity

The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD59 Antibody (MEM-43) - BSA Free

Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).
Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.
Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) [NB500-330] - Human tonsil frozen tissue section stained for CD59 (red) and counterstained with DAPI (blue). Image from verified customer review.
View 6 more images

Applications for CD59 Antibody (MEM-43) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-4 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50
Application Notes
Use in blocking / neutralizing reported in scientific literature (PMID 24909955). CD59 antibody validated for IHC-F from a verified customer review. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB500-330 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD59

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Alternate Names

CD59, HRF20, MACIF, MIC11, Protectin

Gene Symbol

CD59

Additional CD59 Products

Product Documents for CD59 Antibody (MEM-43) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD59 Antibody (MEM-43) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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