CD6 Antibody (3F7B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32942

Clone 3F7B5 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-32942-0.02mg
NBP2-32942-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F7B5

Concentration

0.2 mg/ml

View all CD6 Antibodies »

Product Summary for CD6 Antibody (3F7B5)

Immunogen

Human rheumatoid synovial T cell line ST-1

Localization

Cell surface and cytoplasmic

Marker

Negative Marker of T-regulatory Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34537)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD6 Antibody (3F7B5)

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) [NBP2-32942]

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) [NBP2-32942]

Immunocytochemistry/Immunofluorescence: CD6 Antibody (3F7B5) [NBP2-32942] - Immunofluorescence staining of MOLT-4 cells using CD6 Antibody (3F7B5) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942]

Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942]

Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD6 MAb (3F7B5).
Flow Cytometry: CD6 Antibody (3F7B5) [NBP2-32942]

Flow Cytometry: CD6 Antibody (3F7B5) [NBP2-32942]

Flow Cytometry: CD6 Antibody (3F7B5) [NBP2-32942] - Flow Cytometric Analysis of MOLT-4 cells. CD6 Antibody (3F7B5) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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Applications for CD6 Antibody (3F7B5)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD6

CD6 is a 100-130 kD single chain transmembrane glycoprotein also known as T12. It is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6, interacting with its ligand CD166 (also known as ALCAM), is involved in T cell development and activation, as well as thymocyte adhesion.

Alternate Names

CD6

Gene Symbol

CD6

UniProt

P30203 (Human)

Additional CD6 Products

Product Documents for CD6 Antibody (3F7B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD6 Antibody (3F7B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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