Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM547

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD6 Antibodies »

Product Specifications

Immunogen

Human recombinant CD6 protein (Uniprot: P30203)

Localization

Cell surface and Cytoplasmic

Marker

Negative Marker of T-regulatory Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32828).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD6 Antibody (SPM547) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPM547) - Azide and BSA Free [NBP2-34789]

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPM547) - Azide and BSA Free [NBP2-34789]

Immunocytochemistry/Immunofluorescence: CD6 Antibody (SPM547) - Azide and BSA Free [NBP2-34789] - Immunofluorescence staining of MOLT-4 cells using CD6 Antibody (SPM547) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) - Azide and BSA Free [NBP2-34789]

Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) - Azide and BSA Free [NBP2-34789]

Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) - Azide and BSA Free [NBP2-34789] - Formalin-fixed paraffin-embedded human tonsil stained with CD6 Monoclonal antibody (SPM547).

Applications for CD6 Antibody (SPM547) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD6

CD6 is a 100-130 kD single chain transmembrane glycoprotein also known as T12. It is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6, interacting with its ligand CD166 (also known as ALCAM), is involved in T cell development and activation, as well as thymocyte adhesion.

Alternate Names

CD6

Gene Symbol

CD6

Additional CD6 Products

Product Documents for CD6 Antibody (SPM547) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD6 Antibody (SPM547) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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