CD63 Antibody (H5C6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42225

Clone H5C6 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-42225
NBP2-42225SS
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Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human, Mouse, Rat, Canine

Applications

Validated:

Block/Neutralize, Dot Blot, Electron Microscopy, ELISA, Flow (Intracellular), Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Whole-Mount, Immunoprecipitation, In vitro assay, Western Blot

Cited:

Block/Neutralize, Cytometric Bead Assay Standard, Dot Blot, Electron Microscopy, ELISA, Flow Cytometry, Functional Assay, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # H5C6

Format

BSA Free

Concentration

1.0 mg/ml

View all CD63 Antibodies »

Product Specifications

Immunogen

Human splenic adherent cells.

Marker

Exosome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD63 Antibody (H5C6) - BSA Free

Western Blot: CD63 Antibody (H5C6)BSA Free [NBP2-42225]

Western Blot: CD63 Antibody (H5C6)BSA Free [NBP2-42225]

Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - THP1 whole cell protein was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with anti-CD63 antibody at 2 ug/mL and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) - BSA Free [NBP2-42225]

Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) - BSA Free [NBP2-42225]

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - The CD63 (H5C6) antibody was tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) - BSA Free [NBP2-42225]

Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) - BSA Free [NBP2-42225]

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to DyLight 550 (NBP2-42225R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
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Applications for CD63 Antibody (H5C6) - BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 35602933)

Electron Microscopy

reported in scientific literature (PMID 16735575)

Flow Cytometry

1:1000

Functional

reported in scientific literature (PMID 9811687)

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry Whole-Mount

reported in scientific literature (PMID 21464080)

In vitro assay

reported in scientific literature (PMID 21464080)

Reviewed Applications

Read 6 reviews rated 3.7 using NBP2-42225 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD63

CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface in the endosomal system (1-3). More specifically, it is generally present in multivesicular bodies (MVBs), also called late endosomes, and lysosomes (1). The CD63 molecule is a total of 238 amino acids (aa), has four hydrophobic membrane-spanning regions and three N-glycosylation sites, and the encoded protein has a theoretical molecular weight of 25 kDa (2,3). CD63 both directly and indirectly interacts with other proteins including integrins, cell surface receptors, other tetraspanins, kinases, and adapter proteins, to name a few (1). CD63 is involved in many cell processes including cell survival and activation, cell adhesion, invasion, and migration (1). Additionally, CD63 has been shown to interact with tissue inhibitor of metalloproteinase 1 (TIMP-1), which originally thought to be an inhibitor of cancer progression has recently been shown to have cancer promoting properties as well (1,4). The CD63-TIMP-1 interaction has been shown to activate the PI3K/AKT pathway in lung adenocarcinoma cells and also promote survival and invasion of acute myeloid leukemia cells (1).CD63 is a useful flow cytometry marker for basophil granulocytes and can be used for the basophil activation test (BAT) to assess IgE-mediated allergy response (5). As CD63 was initially discovered on activated blood platelets and is a lysosomal-associated protein it makes sense it would be involved in platelet or lysosomal-related disorders (1,2). More precisely, CD63 is associated with Hermansky-Pudlak syndrome, a disease characterized by albinism and platelet storage pool deficiency (6).

References

1. Pols, M. S., & Klumperman, J. (2009). Trafficking and function of the tetraspanin CD63. Experimental cell research. https://doi.org/10.1016/j.yexcr.2008.09.020

2. Metzelaar, M. J., Wijngaard, P. L., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., & Clevers, H. C. (1991). CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The Journal of biological chemistry.

3. Horejsi, V., & Vlcek, C. (1991). Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS letters. https://doi.org/10.1016/0014-5793(91)80988-f

4. Eckfeld, C., HauBler, D., Schoeps, B., Hermann, C. D., & Kruger, A. (2019). Functional disparities within the TIMP family in cancer: hints from molecular divergence. Cancer metastasis reviews. https://doi.org/10.1007/s10555-019-09812-6

5. Hoffmann, H. J., Santos, A. F., Mayorga, C., Nopp, A., Eberlein, B., Ferrer, M., Rouzaire, P., Ebo, D. G., Sabato, V., Sanz, M. L., Pecaric-Petkovic, T., Patil, S. U., Hausmann, O. V., Shreffler, W. G., Korosec, P., & Knol, E. F. (2015). The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy. https://doi.org/10.1111/all.12698

6. Dell'Angelica, E. C., Shotelersuk, V., Aguilar, R. C., Gahl, W. A., & Bonifacino, J. S. (1999). Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. Molecular cell. https://doi.org/10.1016/s1097-2765(00)80170-7

Alternate Names

CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30

Entrez Gene IDs

967 (Human)

Gene Symbol

CD63

UniProt

P08962

Additional CD63 Products

Product Documents for CD63 Antibody (H5C6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD63 Antibody (H5C6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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