CD68/SR-D1 Antibody (514H12)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65101

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-65101-0.025ml
NB100-65101
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot (Negative)

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 514H12

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CD68/SR-D1 Antibodies »

Product Specifications

Immunogen

Fusion protein corresponding to external domain of human CD68

Specificity

This CD68/SR-D1 Antibody (514H12) recognizes the human CD68 cell surface antigen

Marker

Histiocytic Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CD68/SR-D1 Antibody (514H12)

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (514H12) [NB100-65101]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (514H12) [NB100-65101]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (514H12) [NB100-65101] - Detection of Mouse anti Human CD68 (NB100-65101) in an immunohistochemical analysis of formalin fixed, paraffin embedded tonsil section following heat mediated antigen retrieval with TRIS-EDTA buffer, pH9.0 using the Histar Detection Kit.

Applications for CD68/SR-D1 Antibody (514H12)

Application
Recommended Usage

Immunohistochemistry

1:40 - 1:80

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:40-1:80
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH8.0 is recommended for this purpose. Does not work in Western Blotting.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue Culture Supernatant

Preservative

0.01% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD68/SR-D1

Human CD68, also known as GP110, LAMP4, Scavenger Receptor D1 (SR-D1) or macrosialin in mouse, encodes a 110-kD transmembrane glycoprotein that belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. Members of the LAMP family include LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (aka CD208), and brain and dendritic cell-associated (BAD)-LAMP (aka LAMP-5). Unlike the two LAMP domains facing the lysosomal lumen in LAMP-1 and LAMP-2, CD68 has a single LAMP domain containing four cystines spaced 36-37 residues apart along with an N-terminal Mucin-like domain. The 354 amino acid (a.a.) human CD68 and 326 a.a. murine ortholog share 80.6% a.a. sequence identity (1).

CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).

Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).

References

1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795

2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Entrez Gene IDs

968 (Human)

Gene Symbol

CD68

UniProt

P34810

Additional CD68/SR-D1 Products

Product Documents for CD68/SR-D1 Antibody (514H12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD68/SR-D1 Antibody (514H12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.