Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Armenian Hamster IgG1 Clone # H1.2F3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD69 Antibodies »

Product Specifications

Immunogen

Mouse dendritic epidermal cell line Y245

Reactivity Notes

Mouse reactivity reported in (PMID: 29367423).

Specificity

Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 kDa (unreduced)

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG1

Scientific Data Images for CD69 Antibody (H1.2F3) [PE]

CD69 Antibody (H1.2F3) [PE]

CD69 Antibody (H1.2F3) [PE] [NBP1-28011PE] -

CD69 Antibody (H1.2F3) [PE] [NBP1-28011PE] - Vial of PE conjugated antibody. PE has two excitation maxima, 498 nm excited by the Blue laser (488 nm) and 565 nm excited by the Yellow-Green laser (561 nm). Both result in emission at 578 nm.

Applications for CD69 Antibody (H1.2F3) [PE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD69

CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. (1,2) It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. (3) CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. (1-4) Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils. (4) Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. (4,5) MAb H1.2F3 augments PMA-induced T-cell proliferation,1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells. (7)

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Gene Symbol

CD69

Additional CD69 Products

Product Documents for CD69 Antibody (H1.2F3) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD69 Antibody (H1.2F3) [PE]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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