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CD7 Antibody (CD7/8357R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20468

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # CD7/8357R

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa 1-200) of human CD7 protein (exact sequence is proprietary)

Localization

Cell Surface

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: HePG-2 or Jurkat cells. Human lymph node or tonsil.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for CD7 Antibody (CD7/8357R)

CD7 Antibody (CD7/8357R)

CD7 Antibody (CD7/8357R)

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using CD7 antibody (CD7/8357R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
CD7 Antibody (CD7/8357R)

CD7 Antibody (CD7/8357R)

Formalin-fixed, paraffin-embedded human lymph node stained with CD7 antibody (CD7/8357R). h HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Applications for CD7 Antibody (CD7/8357R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD7

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Alternate Names

CD7, LEU-9, TP41

Gene Symbol

CD7

Additional CD7 Products

Product Documents for CD7 Antibody (CD7/8357R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD7 Antibody (CD7/8357R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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