Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CerCLIP.1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD74 Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from the sequence of human CLIP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD74 Antibody (CerCLIP.1)

Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249]

Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249]

Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249] - A cell surface stain was performed on Daudi cells with CD74 Antibody (CerCLIP.1) NBP1-19249AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for CD74 Antibody (CerCLIP.1)

Application
Recommended Usage

Flow Cytometry

1:200

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD74

CLIP (class II-associated invariant chain peptide) is an important peptide in the MHC-II antigen processing pathway, formed by the enzymatic cleavage of amino acids 81-104 from the invariant chain. During MHC-II synthesis in the ER and transport to endocytic vesicles, the antigen binding site of MHC-II is obstructed by the presence of the invariant chain. As the MHC-II complex moves through the Golgi, the invariant chain is degraded, leaving the CLIP fragment in the MHC-II peptide binding groove. CLIP is eventually displaced by the loading of antigenic peptides, followed by presentation on the APC cell surface to CD4+ T-cells.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Entrez Gene IDs

972 (Human)

Gene Symbol

CD74

Additional CD74 Products

Product Documents for CD74 Antibody (CerCLIP.1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD74 Antibody (CerCLIP.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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