Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD74 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CD74(NP_001020330) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD74 Antibody (OTI1H3) - Azide and BSA Free

Western Blot: CD74 Antibody (OTI1H3)Azide and BSA Free [NBP2-70382]

Western Blot: CD74 Antibody (OTI1H3)Azide and BSA Free [NBP2-70382]

Western Blot: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74.
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382]

Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382]

Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382]

Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382]

Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for CD74 Antibody (OTI1H3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD74

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41 kDa, depending on genetic splicing.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Gene Symbol

CD74

Additional CD74 Products

Product Documents for CD74 Antibody (OTI1H3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD74 Antibody (OTI1H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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