CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # PIN.1

Format

BSA Free

Concentration

1.0 mg/ml

View all CD74 Antibodies »

Product Specifications

Immunogen

Peptide corresponding to residues 12-27 of CD74, human invariant chain (short form).

Epitope

The epitope is in a region of the cytoplasmic tail of human CD74 which is common to all isoforms.

Reactivity Notes

Human. Does not react with mouse.

Localization

Type II membrane protein

Specificity

This detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for CD74 Antibody (PIN.1) - BSA Free

Western Blot: CD74 Antibody (PIN.1)BSA Free [NB100-1985]

Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - BSA Free [NB100-1985]

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.

Flow Cytometry: CD74 Antibody (PIN.1) - BSA Free [NB100-1985]

Flow Cytometry: CD74 Antibody (PIN.1) [NB100-1985] - CD74 in human PBMCs (gated on all monocytes). Orange represents isotype control and blue represents anti-CD74 antibody staining.

Western Blot: CD74 Antibody (PIN.1)BSA Free [NB100-1985]

Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Ramos whole cell protein was separated on a 4-12% gel by SDS-PAGE and protein transferred to PVDF. The membrane was probed with anti-CD74 at 2 ug/ml and detected with and anti-mouse HRP secondary antibody using chemiluminescence. The arrow head shows the detection of CD74.

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - BSA Free [NB100-1985]

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - BSA Free [NB100-1985]

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.

CD74 (PIN.1) in U-2 OS Human Cell Line -

CD74 (PIN.1) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-CD74 (PIN.1) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-1985AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

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Applications for CD74 Antibody (PIN.1) - BSA Free

Application

Recommended Usage

Flow Cytometry

1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1 ug/ml

Application Notes

In Simple Western only 10-15 ul of the recommended dilution is used per data point. In WB this detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74. This antibody is CyTOF ready. See Simple Western Antibody Database for Simple Western validation: tested in Jurkat lysate (0.2 mg/ml); antibody dilution of 1:400; separated by size; detects a band at 62 kDa

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD74

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41kDa, depending on genetic splicing.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Entrez Gene IDs

972 (Human)

Gene Symbol

CD74

UniProt

P04233 (Human)

Additional CD74 Products

Product Documents for CD74 Antibody (PIN.1) - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download IHC-P Protocol

COA

SDS

Download SDS

Product Specific Notices for CD74 Antibody (PIN.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CD74 Antibody (PIN.1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1985

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all CD74 Antibodies »

Product Specifications

Immunogen

Epitope

Reactivity Notes

Localization

Specificity

Clonality

Host

Isotype

Scientific Data Images for CD74 Antibody (PIN.1) - BSA Free

Western Blot: CD74 Antibody (PIN.1)BSA Free [NB100-1985]

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - BSA Free [NB100-1985]

Flow Cytometry: CD74 Antibody (PIN.1) - BSA Free [NB100-1985]

Western Blot: CD74 Antibody (PIN.1)BSA Free [NB100-1985]

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - BSA Free [NB100-1985]

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - BSA Free [NB100-1985]

CD74 (PIN.1) in U-2 OS Human Cell Line -

Applications for CD74 Antibody (PIN.1) - BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CD74

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CD74 Products

Product Documents for CD74 Antibody (PIN.1) - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for CD74 Antibody (PIN.1) - BSA Free