CD79A Antibody (HM57)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64347

Clone HM57 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Avian - Chicken, Bovine, Canine

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HM57

Concentration

1.0 mg/ml

View all CD79A Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1

Reactivity Notes

Predicted cross-reactivities: Equine, Monkey, Guinea Pig, Goat, Fallow deer, Ferret, Dog, Bovine, American Bison, Porcine, Rabbit, Mouse, Rat, Red deer

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

NB100-64347 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Clone HM57 has been reported to work in western blotting applications.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD79A Antibody (HM57)

Immunohistochemistry: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Immunohistochemistry: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Immunohistochemistry: CD79A Antibody (HM57) [NB100-64347] - Paraffin-embedded alcohol fixed Rat Spleen tissue (20x). Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4C. This image was submitted via customer revies.
Flow Cytometry: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Flow Cytometry: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Flow Cytometry: CD79A Antibody (HM57) [NB100-64347] - Using the Allophycocyanin direct conjugate An intracellular stain was performed on Daudi cells with CD79A (HM 57) antibody NB100-64347APC (blue) and a matched isotype control NBP1-97005APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 0.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Immunohistochemistry-Paraffin: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Immunohistochemistry-Paraffin: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Immunohistochemistry-Paraffin: CD79A Antibody (HM57) [NB100-64347] - Embedded human tonsil.

Applications for CD79A Antibody (HM57)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: heat treated antigen retrieval using sodium citrate buffer pH 6.0 is recommended for this purpose. Although not tested this antibody may be useful in Western Blot. NB100-64347 has been used successfully in Immunofluorescence reported by a by a customer review.

Reviewed Applications

Read 6 reviews rated 3.5 using NB100-64347 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD79A

A disulfide-linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, is non-covalently associated with membrane bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling.

Alternate Names

CD79A, MB-1

Entrez Gene IDs

973 (Human); 12518 (Mouse); 681236 (Rat)

Gene Symbol

CD79A

UniProt

P11912

Additional CD79A Products

Product Documents for CD79A Antibody (HM57)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD79A Antibody (HM57)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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