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CD79A Antibody (rIGA/6986)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20524

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rIGA/6986

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa1-100) of human CD79A protein (exact sequence is proprietary)

Localization

Cell Surface

Specificity

This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20 Anti-CD79A also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Positive Controls: Germinal center B-cells in a lymph node or tonsil.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for CD79A Antibody (rIGA/6986)

Western blot analysis of Daudi cell lysate using CD79A antibody (rIGA/6986).
CD79A Antibody (rIGA/6986)

CD79A Antibody (rIGA/6986)

Formalin-fixed, paraffin-embedded human tonsil stained with CD79A antibody (rIGA/6986). Inset: PBS instead of primary antibody; secondary only negative control.
CD79A Antibody (rIGA/6986)

CD79A Antibody (rIGA/6986)

Formalin-fixed, paraffin-embedded human tonsil stained with CD79A antibody (rIGA/6986). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for CD79A Antibody (rIGA/6986)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD79A

CD79a is a disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines.

Alternate Names

CD79A, MB-1

Gene Symbol

CD79A

Additional CD79A Products

Product Documents for CD79A Antibody (rIGA/6986)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD79A Antibody (rIGA/6986)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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