CD81 Antibody (M38) [Janelia Fluor® 525]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44861JF525

Novus Biologicals, part of Bio-Techne
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NBP1-44861
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Feline, Rabbit

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Single Cell Western, Western Blot

Label

Janelia Fluor 525

Antibody Source

Monoclonal Mouse IgG1 Clone # M38

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD81 Antibodies »

Product Summary for CD81 Antibody (M38) [Janelia Fluor® 525]

Immunogen

MOLT-4, human T-ALL cell line (NM_004356.3).

Reactivity Notes

Cat (100%). Use in Mouse reported in scientific publication( PMID: 32573489).

Specificity

The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD81 Antibody (M38) [Janelia Fluor® 525]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Single Cell Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD81

CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. (1) CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors. (2) Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha. (2) The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. (2)

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for CD81 Antibody (M38) [Janelia Fluor® 525]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD81 Antibody (M38) [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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