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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Bovine

Predicted:

Bovine (95%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Electron Microscopy, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81. The immunogen is located within amino acids 30 - 80 of CD81. Amino Acid Squence: HDPQTTNLLYLELGDKPAPN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD81 Antibody - BSA Free

Western Blot: CD81 AntibodyBSA Free [NBP1-77039]

Western Blot: CD81 AntibodyBSA Free [NBP1-77039]

Western Blot: CD81 Antibody [NBP1-77039] - Analysis in human lung tissue lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking petide.
CD81 Antibody - BSA Free

Immunohistochemistry: CD81 Antibody - BSA Free [NBP1-77039] -

Immunohistochemistry: CD81 Antibody - BSA Free [NBP1-77039] - Immunohistochemistry of CD81 in human lung tissue with CD81 antibody at 5 u/mL.
CD81 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CD81 Antibody - BSA Free [NBP1-77039] -

Immunocytochemistry/ Immunofluorescence: CD81 Antibody - BSA Free [NBP1-77039] - Immunofluorescence of CD81 in human brain tissue with CD81 antibody at 10 u/ml.

Green: CD81 Antibody
Blue: DAPI staining

Applications for CD81 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

2.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD81

CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family, a group of cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that associates with integrins and appears to promote muscle cell fusion and support myotube maintenance. Along with SCARB1, LDL-R, and CLDN1, CD81 has been reported to be an entry factor for the Hepatitis C virus. Finally, recent evidence indicates that the CD81 gene is localized in a tumor-suppressor gene region and is thus a candidate gene for malignancies.

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

UniProt

Additional CD81 Products

Product Documents for CD81 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD81 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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