CD81 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80701

Novus Biologicals, part of Bio-Techne
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NBP1-80701
NBP1-80701-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD81 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGKLYL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD81 Antibody

Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701]

Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701]

Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701] - Analysis in human testis and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: CD81 Antibody [NBP1-80701]

Immunocytochemistry/ Immunofluorescence: CD81 Antibody [NBP1-80701]

Immunocytochemistry/Immunofluorescence: CD81 Antibody [NBP1-80701] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701]

Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701]

Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
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Applications for CD81 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD81

CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. (1) CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors. (2) Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha. (2) The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. (2)

Alternate Names

CD81, TAPA-1, TSPAN28

Entrez Gene IDs

975 (Human)

Gene Symbol

CD81

UniProt

P60033

Additional CD81 Products

Product Documents for CD81 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD81 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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