CD81 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20564

Novus Biologicals, part of Bio-Techne
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NBP2-20564
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Chimpanzee (100%), Porcine (92%), Rhesus Macaque (96%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Validated:

Block/Neutralize, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD81 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.

Reactivity Notes

Mouse (83%), Rat (86%), Bovine (89%).

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD81 Antibody

CD81 Antibody

Western Blot: CD81 Antibody [NBP2-20564] -

Western Blot: CD81 Antibody [NBP2-20564] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CD81 Antibody [NBP2-20564]

Immunohistochemistry-Paraffin: CD81 Antibody [NBP2-20564]

Immunohistochemistry-Paraffin: CD81 Antibody [NBP2-20564] - CD81 Antibody Immunohistochemical analysis of paraffin-embedded Lung Papillary Adenocarcinoma, using antibody at 1:250 dilution.
Flow Cytometry: CD81 Antibody [NBP2-20564]

Flow Cytometry: CD81 Antibody [NBP2-20564]

Flow Cytometry: CD81 Antibody [NBP2-20564] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD81 antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
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Applications for CD81 Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CD81

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq]

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

UniProt

P60033

Additional CD81 Products

Product Documents for CD81 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD81 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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