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CD82/Kai-1 Antibody (C33) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21792

Novus Biologicals, part of Bio-Techne
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NBP2-21792
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # C33

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for CD82/Kai-1 Antibody (C33) - BSA Free

Immunogen

C91/PL (human HTLV-1+ T cell line).

Specificity

The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CD82/Kai-1 Antibody (C33) - BSA Free

Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]

Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]

Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).
Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]

Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]

Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) PE.
Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]

Flow Cytometry: CD82/Kai-1 Antibody (C33) - BSA Free [NBP2-21792]

Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.

Applications for CD82/Kai-1 Antibody (C33) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:50-1:300

Immunohistochemistry

1:50-1:300

Immunohistochemistry-Paraffin

1:50-1:300

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:2000
Application Notes
In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD82/Kai-1

CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.

Alternate Names

CD82, IA4, KAI1, SAR2, ST6, TSPAN27

Entrez Gene IDs

3732 (Human)

Gene Symbol

CD82

Additional CD82/Kai-1 Products

Product Documents for CD82/Kai-1 Antibody (C33) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD82/Kai-1 Antibody (C33) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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