CD90/Thy1 Antibody (G7) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28033

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay

Cited:

In vitro assay

Label

Biotin

Antibody Source

Monoclonal Rat IgG2c Kappa Clone # G7

Concentration

0.5 mg/ml

View all CD90/Thy1 Antibodies »

Product Specifications

Immunogen

Mouse T cell hybridoma C6/G8

Specificity

Mouse CD90 (Thy-1.1 & Thy-1.2 alloantigens/Thy-1 epitope region A); Mr 28-32 kDa

Marker

Mesenchymal Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2c Kappa

Scientific Data Images for CD90/Thy1 Antibody (G7) [Biotin]

Flow Cytometry: CD90/Thy1 Antibody (G7) [Biotin] [NBP1-28033] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-BIOT and Rat Anti-Mouse CD3 epsilon-PE followed by Streptavidin-FITC.

Applications for CD90/Thy1 Antibody (G7) [Biotin]

Application
Recommended Usage

Flow Cytometry

<= 1 ug/10^6 cells

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD90/Thy1

CD90/Thy-1, a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. (1-4) It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes and neurons of all mouse strains. (1,2) MAb G7 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. (1,5-7) The MAb has also been reported to promote apoptosis of thymocytes and CTL clones,4,7 and to mediate adhesion of thymocytes to thymic stroma.8

Alternate Names

CD90, Thy1

Gene Symbol

THY1

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (G7) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD90/Thy1 Antibody (G7) [Biotin]

Conjugated forms should be protected from prolonged exposure to light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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