CD90/Thy1 Antibody (JF10-09)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66763
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JF10-09
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human CD90/Thy1 aa 75-120 / 161. (SwissProt: P04216 Human; SwissProt: P01831 Mouse; SwissProt: P01830 Rat)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CD90/Thy1 Antibody (JF10-09)
Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763]
Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVECImmunocytochemistry/ Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763]
Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763]
Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for CD90/Thy1 Antibody (JF10-09)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Frozen
1:100
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD90/Thy1
Alternate Names
CD90, Thy1
Gene Symbol
THY1
Additional CD90/Thy1 Products
Product Documents for CD90/Thy1 Antibody (JF10-09)
Product Specific Notices for CD90/Thy1 Antibody (JF10-09)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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