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CD98 Antibody (RL388) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12036

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RL388

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

This antibody was raised by immunising rats with an IL 2-producing subline of the mouse T lymphoma EL4.

Specificity

This antibody recognises murine CD98, a heterodimeric cell surface glycoprotein.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD98 Antibody (RL388) - Chimeric - Azide and BSA Free

Western Blot: CD98 Antibody (RL388)ChimericAzide and BSA Free [NBP3-12036]

Western Blot: CD98 Antibody (RL388)ChimericAzide and BSA Free [NBP3-12036]

Western Blot: CD98 Antibody (RL388) - Chimeric [NBP3-12036] - Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (NBP3-12036) at 0.05 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CD98 Antibody (RL388) - Chimeric - Azide and BSA Free [NBP3-12036]

Immunocytochemistry/ Immunofluorescence: CD98 Antibody (RL388) - Chimeric - Azide and BSA Free [NBP3-12036]

Immunocytochemistry/Immunofluorescence: CD98 Antibody (RL388) - Chimeric [NBP3-12036] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RL388 (NBP3-12036) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12036, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.

Applications for CD98 Antibody (RL388) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD98

The CD98 gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible

Alternate Names

4F2, 4F2hc, CD98, CH98hc, FRP-1, SLC3A2

Gene Symbol

SLC3A2

Additional CD98 Products

Product Documents for CD98 Antibody (RL388) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD98 Antibody (RL388) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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