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CD99 Antibody (MEM-131) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-622

Clone MEM-131 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB500-622-100Tests

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgM Clone # MEM-131

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD99 Antibody (MEM-131) [FITC]

Immunogen

HPB-ALL human peripheral blood leukemia T-cell line

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Specificity

The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes. HLDA V; WS Code AS S020 HLDA V; WS Code T T-E2.02 HLDA V; WS Code T T-017

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for CD99 Antibody (MEM-131) [FITC]

Flow Cytometry: CD99 Antibody (MEM-131) [FITC] [NB500-622] - Surface staining of human peripheral blood with anti-CD99R (MEM-131) FITC.

Applications for CD99 Antibody (MEM-131) [FITC]

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

TBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD99

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

Additional CD99 Products

Product Documents for CD99 Antibody (MEM-131) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD99 Antibody (MEM-131) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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