CD99 Antibody (SPM586)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44693
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # SPM586
Concentration
0.2 mg/ml
Product Summary for CD99 Antibody (SPM586)
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes
Localization
Cell surface
Specificity
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors. This monoclonal antibody shows a very similar reactivity to other CD99 monoclonal antibodys (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Marker
Ewing's Sarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
200ug/ml of antibody purified by Protein L. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11457)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD99 Antibody (SPM586)
Immunohistochemistry-Paraffin: CD99 Antibody (SPM586) [NBP2-44693]
Immunohistochemistry-Paraffin: CD99 Antibody (SPM586) [NBP2-44693] - Human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).Applications for CD99 Antibody (SPM586)
Application
Recommended Usage
Flow Cytometry
5-10ul/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
This antibody is CyTOF ready.
Optimal dilution for a specific application should be determined.
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein L purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD99
Additional CD99 Products
Product Documents for CD99 Antibody (SPM586)
Product Specific Notices for CD99 Antibody (SPM586)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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