CD99 Antibody (SPM596)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44696
![Immunohistochemistry-Paraffin: CD99 Antibody (SPM596) [NBP2-44696]](https://resources.bio-techne.com/images/products/CD99-Antibody-SPM596-Immunohistochemistry-Paraffin-NBP2-44696-img0001.jpg "Immunohistochemistry-Paraffin: CD99 Antibody (SPM596) [NBP2-44696]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM596
Concentration
0.2 mg/ml
Product Summary for CD99 Antibody (SPM596)
Immunogen
Recombinant full-length human CD99 protein (Uniprot: P14209)
Localization
Cell surface
Marker
Ewing's Sarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11459)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD99 Antibody (SPM596)
Immunohistochemistry-Paraffin: CD99 Antibody (SPM596) [NBP2-44696]
Immunohistochemistry-Paraffin: CD99 Antibody (SPM596) [NBP2-44696] - Human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (SPM596).Applications for CD99 Antibody (SPM596)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD99
Additional CD99 Products
Product Documents for CD99 Antibody (SPM596)
Product Specific Notices for CD99 Antibody (SPM596)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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