Key Product Details

Species Reactivity

Validated:

Human, Bovine, Drosophila (Negative), Mink, Monkey, Mouse (Negative), Rat (Negative), Xenopus (Negative)

Cited:

Bovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # POH-1; same as cdc2.1

Concentration

0.2 mg/ml

View all CDC2/CDK1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human p34cdc2 protein (Uniprot: P06493)

Reactivity Notes

Does not react with Mouse, Rat, Xenopus, drosophila, S, pombe or S. cerevisiae.

Localization

Nuclear and Cytoplasmic

Specificity

Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34711)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CDC2/CDK1 Antibody (POH-1; same as cdc2.1)

Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331]

Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331]

Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Analysis using the Azide and BSA Free version of NBP2-34331. Staining of human Tonsil stained with Cdk1 MAb (POH-1).

Applications for CDC2/CDK1 Antibody (POH-1; same as cdc2.1)

Application
Recommended Usage

Immunohistochemistry-Paraffin

2-4 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Gene Symbol

CDK1

UniProt

P06493 (Human)

Additional CDC2/CDK1 Products

Product Documents for CDC2/CDK1 Antibody (POH-1; same as cdc2.1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDC2/CDK1 Antibody (POH-1; same as cdc2.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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