CDC2/CDK1 [p Tyr15] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-142

Novus Biologicals, part of Bio-Techne
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NB500-142
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Xenopus

Predicted:

Mouse (100%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC2/CDK1 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH. Accession # P39951

Modification

p Tyr15

Localization

Nuclear

Specificity

Specific for endogenous levels of the ~34 kDa CDC2/CDK1 protein phosphorylated at Tyr15. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDC2/CDK1 [p Tyr15] Antibody

Western Blot: CDC2/CDK1 [p Tyr15] Antibody [NB500-142]

Western Blot: CDC2/CDK1 [p Tyr15] Antibody [NB500-142]

Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).

Applications for CDC2/CDK1 [p Tyr15] Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
NB500-142 can be used in Western blot where a band is seen at ~ 34 kDa, representing CDC2. Immunohistochemical analysis has not yet been determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB500-142 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Entrez Gene IDs

983 (Human); 12534 (Mouse); 54237 (Rat)

Gene Symbol

CDK1

UniProt

Q5XI98 (Rat)

Additional CDC2/CDK1 Products

Product Documents for CDC2/CDK1 [p Tyr15] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CDC2/CDK1 [p Tyr15] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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