CDC2/CDK1 [p Tyr15] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB500-142
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Xenopus
Predicted:
Mouse (100%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH. Accession # P39951
Modification
p Tyr15
Localization
Nuclear
Specificity
Specific for endogenous levels of the ~34 kDa CDC2/CDK1 protein phosphorylated at Tyr15. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CDC2/CDK1 [p Tyr15] Antibody
Western Blot: CDC2/CDK1 [p Tyr15] Antibody [NB500-142]
Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).Applications for CDC2/CDK1 [p Tyr15] Antibody
Application
Recommended Usage
Western Blot
1:1000
Application Notes
NB500-142 can be used in Western blot where a band is seen at ~ 34 kDa, representing CDC2. Immunohistochemical analysis has not yet been determined.
Reviewed Applications
Read 1 review rated 5 using NB500-142 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CDC2/CDK1
Long Name
Cell Division Cycle 2/Cyclin-dependent Kinase 1
Alternate Names
CDK1
Gene Symbol
CDK1
UniProt
Additional CDC2/CDK1 Products
Product Documents for CDC2/CDK1 [p Tyr15] Antibody
Product Specific Notices for CDC2/CDK1 [p Tyr15] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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