CDC2/CDK1 [p Thr14] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13300

Novus Biologicals, part of Bio-Techne
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NBP3-13300
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Chimpanzee (100%), Drosophila (100%), Fish (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Sheep (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDC2/CDK1 Antibodies »

Product Summary for CDC2/CDK1 [p Thr14] Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr14 of human CDC2/CDK1. The exact sequence is proprietary.

Modification

p Thr14

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CDC2/CDK1 [p Thr14] Antibody

Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300]

Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300]

Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC2/CDK1 (phospho Thr14) antibody (NBP3-13300) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300]

Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300]

Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC2/CDK1 (phospho Thr14) antibody (NBP3-13300) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for CDC2/CDK1 [p Thr14] Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Gene Symbol

CDK1

Additional CDC2/CDK1 Products

Product Documents for CDC2/CDK1 [p Thr14] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDC2/CDK1 [p Thr14] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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