Cdc20 Antibody (AR12) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34552
![Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) - Azide and BSA Free [NBP2-34552]](https://resources.bio-techne.com/images/products/Cdc20-Antibody-AR12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34552-img0001.jpg "Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) - Azide and BSA Free [NBP2-34552]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # AR12
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Cdc20 Antibody (AR12) - Azide and BSA Free
Immunogen
Urea-denatured His6 Cdc20 human recombinant protein (Uniprot: Q12834)
Localization
Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32954).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cdc20 Antibody (AR12) - Azide and BSA Free
Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) - Azide and BSA Free [NBP2-34552]
Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) - Azide and BSA Free [NBP2-34552] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Cdc20 Antibody (AR12).![Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) - Azide and BSA Free [NBP2-34552]](https://resources.bio-techne.com/images/products/Cdc20-Antibody-AR12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34552-img0002.jpg "Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) - Azide and BSA Free [NBP2-34552]")
Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) - Azide and BSA Free [NBP2-34552]
Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) - Azide and BSA Free [NBP2-34552] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cdc20 Antibody (AR12).Applications for Cdc20 Antibody (AR12) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cdc20
Long Name
Cell division cycle protein 20 homolog
Alternate Names
p55CDC
Gene Symbol
CDC20
Additional Cdc20 Products
Product Documents for Cdc20 Antibody (AR12) - Azide and BSA Free
Product Specific Notices for Cdc20 Antibody (AR12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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