Cdc20 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-59827

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Cdc20 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human cell division cycle 20 using the numbering given in entry NP_001246.2 (GeneID 991).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cdc20 Antibody

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:500 (2ug/ml). Detection: DAB.
Immunoprecipitation: Cdc20 Antibody [NB100-59827]

Immunoprecipitation: Cdc20 Antibody [NB100-59827]

Immunoprecipitation: Cdc20 Antibody [NB100-59827] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59827 (lot 2) used for IP at 6 ug/mg lysate. CDC20 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated CDC20, NB100-59827 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Cdc20 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200- 1:1000

Immunohistochemistry-Paraffin

1:200- 1:1000

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cdc20

CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.

Long Name

Cell division cycle protein 20 homolog

Alternate Names

p55CDC

Entrez Gene IDs

991 (Human)

Gene Symbol

CDC20

UniProt

Q12834 (Human)

Additional Cdc20 Products

Product Documents for Cdc20 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cdc20 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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