Cdc20 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38750

Novus Biologicals, part of Bio-Techne
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NBP2-38750
NBP2-38750-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cdc20 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdc20 Antibody

Immunohistochemistry-Paraffin: Cdc20 Antibody [NBP2-38750]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NBP2-38750]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NBP2-38750] - Analysis in human testis and skeletal muscle tissues. Corresponding CDC20 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Cdc20 Antibody [NBP2-38750]

Immunocytochemistry/ Immunofluorescence: Cdc20 Antibody [NBP2-38750]

Immunocytochemistry/Immunofluorescence: Cdc20 Antibody [NBP2-38750] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cdc20 Antibody [NBP2-38750]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NBP2-38750]

Immunohistochemistry-Paraffin: Cdc20 Antibody [NBP2-38750] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in enteroendocrine cells.
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Applications for Cdc20 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cdc20

CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.

Long Name

Cell division cycle protein 20 homolog

Alternate Names

p55CDC

Gene Symbol

CDC20

UniProt

Q12834 (Human)

Additional Cdc20 Products

Product Documents for Cdc20 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cdc20 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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