CDC25C Antibody (3B11)
Novus Biologicals, part of Bio-Techne | Catalog # H00000995-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3B11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKCCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPAQLLCST
Specificity
CDC25C - cell division cycle 25C
Marker
Mitosis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for CDC25C Antibody (3B11)
Western Blot: CDC25C Antibody (3B11) [H00000995-M01]
Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.Lane 1: CDC25C transfected lysate(53.312 KDa).Lane 2: Non-transfected lysate.Immunohistochemistry-Paraffin: CDC25C Antibody (3B11) [H00000995-M01]
Immunohistochemistry-Paraffin: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 6 ug/ml.Western Blot: CDC25C Antibody (3B11) [H00000995-M01]
Western Blot: CDC25C Antibody (3B11) [H00000995-M01] - Analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi ( Cat # H00000995-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody (M01), clone 3B11 (Cat # H00000995-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for CDC25C Antibody (3B11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CDC25C
Long Name
Cell Division Cycle 25C
Alternate Names
CDC 25, CDC25, cell division cycle 25 homolog C (S. cerevisiae), cell division cycle 25 homolog C (S. pombe), cell division cycle 25C, Dual specificity phosphatase Cdc25C, EC 3.1.3.48, mitosis inducer CDC25, M-phase inducer phosphatase 3, phosphotyrosine phosphatase
Entrez Gene IDs
995 (Human)
Gene Symbol
CDC25C
OMIM
157680 (Human)
UniProt
Additional CDC25C Products
Product Documents for CDC25C Antibody (3B11)
Product Specific Notices for CDC25C Antibody (3B11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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