CDC25C [p Ser216] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-81841
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human CDC25C around the phosphorylation site of serine 216 (S-P-SP-M-P).
Modification
p Ser216
Specificity
Detects endogenous levels of CDC25C only when phosphorylated at serine 216.
Marker
Mitosis Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CDC25C [p Ser216] Antibody
Immunocytochemistry/Immunofluorescence: CDC25C [p Ser216] Antibody [NB100-81841] - Staining of methanol-fixed Hela cells using CDC25C [p Ser216] Antibody.
![Immunohistochemistry-Paraffin: CDC25C [p Ser216] Antibody [NB100-81841]](https://resources.bio-techne.com/images/products/CDC25C-[p-Ser216]-Antibody-Immunohistochemistry-Paraffin-NB100-81841-img0007.jpg "Immunohistochemistry-Paraffin: CDC25C [p Ser216] Antibody [NB100-81841]")
Immunohistochemistry-Paraffin: CDC25C [p Ser216] Antibody [NB100-81841]
Immunohistochemistry-Paraffin: CDC25C [p Ser216] Antibody [NB100-81841] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81841 (left) or the same antibody preincubated with blocking peptide(right).Applications for CDC25C [p Ser216] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:100
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CDC25C
Long Name
Cell Division Cycle 25C
Alternate Names
CDC 25, CDC25, cell division cycle 25 homolog C (S. cerevisiae), cell division cycle 25 homolog C (S. pombe), cell division cycle 25C, Dual specificity phosphatase Cdc25C, EC 3.1.3.48, mitosis inducer CDC25, M-phase inducer phosphatase 3, phosphotyrosine phosphatase
Entrez Gene IDs
995 (Human)
Gene Symbol
CDC25C
UniProt
Additional CDC25C Products
Product Documents for CDC25C [p Ser216] Antibody
Product Specific Notices for CDC25C [p Ser216] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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