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Cdc27 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-465

Novus Biologicals, part of Bio-Techne
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NB600-465

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (100%), Chimpanzee (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This affinity purified Cdc27 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 415-445 of Human Cdc27. (Uniprot: P30260)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, chicken, rat and dog based on 100% homology for the immunogen sequence. Expect cross reactivity with cdc27 from mouse as only a single amino acid residue change is found for the immunogen sequence. Cross reactivity with cdc27 homologues from other sources has not been determined.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity purified antibody is directed against human Cdc27 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Cdc27 Antibody

Western Blot: Cdc27 Antibody [NB600-465]

Western Blot: Cdc27 Antibody [NB600-465]

Western Blot: Cdc27 Antibody [NB600-465] - Analysis of a band 90 kDa corresponding to human cdc27 (arrowhead). Approximately 35 ug of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.

Applications for Cdc27 Antibody

Application
Recommended Usage

ELISA

1:5000-1:40000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kDa in size corresponding to Cdc27 by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Cdc27

Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of naphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Alternate Names

ANAPC3CDC27 homolog, anaphase promoting complex subunit 3, Anaphase-promoting complex subunit 3, anaphase-promoting complex, protein 3, APC 3, APC3cell division cycle 27, CDC27Hs, cell division cycle 27 homolog (S. cerevisiae), cell division cycle protein 27 homolog, D0S1430E, D17S978E, HNUC, H-NUC, NUC2, nuc2 homolog

Entrez Gene IDs

996 (Human)

Gene Symbol

CDC27

UniProt

Additional Cdc27 Products

Product Documents for Cdc27 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cdc27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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