Cdc27 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77377

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human APC3. The immunogen is located within the last 50 amino acids of APC3. Amino Acid Squence: EAIDKRYLPDDEEPITQEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdc27 Antibody - BSA Free

Western Blot: Cdc27 AntibodyBSA Free [NBP1-77377]

Western Blot: Cdc27 AntibodyBSA Free [NBP1-77377]

Western Blot: Cdc27 Antibody [NBP1-77377] - Analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: Cdc27 Antibody - BSA Free [NBP1-77377]

Immunohistochemistry-Paraffin: Cdc27 Antibody - BSA Free [NBP1-77377]

Immunohistochemistry-Paraffin: Cdc27 Antibody [NBP1-77377] - Rat liver tissue with APC3 antibody at 5 ug/mL.
Cdc27 Antibody - BSA Free

Immunohistochemistry: Cdc27 Antibody - BSA Free [NBP1-77377] -

Immunohistochemistry: Cdc27 Antibody - BSA Free [NBP1-77377] - Immunohistochemistry of Cdc27 in rat liver tissue with Cdc27 antibody at 5 u/mL.
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Applications for Cdc27 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cdc27

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1.

Alternate Names

ANAPC3CDC27 homolog, anaphase promoting complex subunit 3, Anaphase-promoting complex subunit 3, anaphase-promoting complex, protein 3, APC 3, APC3cell division cycle 27, CDC27Hs, cell division cycle 27 homolog (S. cerevisiae), cell division cycle protein 27 homolog, D0S1430E, D17S978E, HNUC, H-NUC, NUC2, nuc2 homolog

Gene Symbol

CDC27

UniProt

NP_001247 (Human)

Additional Cdc27 Products

Product Documents for Cdc27 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cdc27 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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