CDC42 Antibody (1V6X7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15619

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15619-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1V6X7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC42 Antibodies »

Product Summary for CDC42 Antibody (1V6X7)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-191 of human CDC42 (P60953). GQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDC42 Antibody (1V6X7)

Western Blot: CDC42 Antibody (1V6X7) [NBP3-15619]

Western Blot: CDC42 Antibody (1V6X7) [NBP3-15619]

Western Blot: CDC42 Antibody (1V6X7) [NBP3-15619] - Western blot analysis of extracts of various cell lines, using CDC42 antibody (NBP3-15619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for CDC42 Antibody (1V6X7)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDC42

CDC42 is a small GTPase of the Rho subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc42, and is able to complement the yeast cdc42 1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott Aldrich syndrome protein (NWASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in at least two transcript variants.

Long Name

Cell Division Cycle 42

Alternate Names

G25K

Gene Symbol

CDC42

Additional CDC42 Products

Product Documents for CDC42 Antibody (1V6X7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CDC42 Antibody (1V6X7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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