CDC42EP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-88140

Novus Biologicals, part of Bio-Techne
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NB100-88140
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CDC42EP3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human binder of Rho GTPases 2 using the numbering given in entry NP_006440.2 (GeneID 10602).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC42EP3 Antibody

Immunoprecipitation: CDC42EP3 Antibody [NB100-88140]

Immunoprecipitation: CDC42EP3 Antibody [NB100-88140]

Immunoprecipitation: CDC42EP3 Antibody [NB100-88140] - Detection of Human BORG2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BORG2 antibody NB100-88140 used for IP at 3 mcg/mg lysate. BORG2 was also immunoprecipitated by rabbit anti-BORG2 antibody NB100-88141 recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for CDC42EP3 Antibody

Application
Recommended Usage

Immunoprecipitation

2- 5 ug/mg lysate
Application Notes
This antibody is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CDC42EP3

CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. This protein can interact with CDC42, as well as with the ras homolog gene family, member Q (ARHQ/TC10). Expression of this protein in fibroblasts has been shown to induce pseudopodia formation. [provided by RefSeq]

Alternate Names

Binder of Rho GTPases 2, BORG2CEP3UB1, CDC42 effector protein (Rho GTPase binding) 3, cdc42 effector protein 3, CRIB-containing BORG2 protein, FLJ46903, MSE55-related Cdc42-binding protein, MSE55-related protein

Entrez Gene IDs

10602 (Human)

Gene Symbol

CDC42EP3

UniProt

NP_006440.2 (Human)

Additional CDC42EP3 Products

Product Documents for CDC42EP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDC42EP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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