CDC5L Antibody (1E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26668
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1E3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human CDC5L [UniProt Q99459]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CDC5L Antibody (1E3)
Immunoprecipitation: CDC5L Antibody (1E3) [NBP3-26668] -
Immunoprecipitation: CDC5L Antibody (1E3) [NBP3-26668] - Immunoprecipitating CDC5L in Hela whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26668 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26668 (3ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (20ug).
Immunohistochemistry: CDC5L Antibody (1E3) [NBP3-26668] -
Immunohistochemistry: CDC5L Antibody (1E3) [NBP3-26668] - Image of CDC5L Antibody (1E3) diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry: CDC5L Antibody (1E3) [NBP3-26668] -
Immunohistochemistry: CDC5L Antibody (1E3) [NBP3-26668] - Image of CDC5L Antibody (1E3) diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.Applications for CDC5L Antibody (1E3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: CDC5L
Alternate Names
CDC5, CDC5 (cell division cycle 5, S. pombe, homolog)-like, CDC5 cell division cycle 5-like (S. pombe), Cdc5-like protein, Cdc5-related protein, CEF1, cell division cycle 5-like protein, dJ319D22.1, dJ319D22.1 (CDC5-like protein), hCDC5, KIAA0432, PCDC5RPCDC5-LIKE, Pombe cdc5-related protein
Gene Symbol
CDC5L
Additional CDC5L Products
Product Documents for CDC5L Antibody (1E3)
Product Specific Notices for CDC5L Antibody (1E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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