CDK2 Antibody (10E0N5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33184
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 10E0N5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 199-298 of human CDK2 (P24941).
Sequence:
RRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
Sequence:
RRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CDK2 Antibody (10E0N5)
Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] -
Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of lysates from wild type(WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] -
Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of various lysates using CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Applications for CDK2 Antibody (10E0N5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CDK2
Long Name
Cyclin-dependent Kinase 2
Alternate Names
cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)
Gene Symbol
CDK2
Additional CDK2 Products
Product Documents for CDK2 Antibody (10E0N5)
Product Specific Notices for CDK2 Antibody (10E0N5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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